Ap Biology Lab Manual Lab 116

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Field of StudyThe AP Biology course is designed to enable students to develop advanced inquiry and reasoning skills, such as designing a plan for collecting data, analyzing data, applying mathematical routines, and connecting concepts in and across domains. The result will be readiness for the study of advanced topics in subsequent college courses.This AP Biology course is equivalent to a two-semester college introductory biology course and has been endorsed enthusiastically by higher education officials.

Completing the Research Notebook for AP Biology Lab #2.Enzyme CatalysisResource: Lab Two, Enzyme CatalysisPage 19 of the AP Biology Lab ManualPart 1: Title (Write the title in the form of a question after you have completed the pre-lab)Part 2: Objectives:The two main objectives are presented in the Overview on the first page of the lab handout (page 19)Part 3: Prelab Questions and Answers:Note: You will need to read the Introduction on pages 19-22 of the lab handout carefully.1. What is a very important role that enzymes have in cells?2. Explain the participants in an “enzyme catalyzed reaction” and explain the “active site” of the enzyme.3. The following are factors that affect an enzyme’s action. For each, explain its effect and why it has this effect.a.

Salt concentration b. Temperature d. Activators and inhibitors.4. Write the equation for the decomposition of hydrogen peroxide.

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Briefly describe catalase and its role in livingcells.5. Read pg 21 and examine Figure 2.1 carefully.

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Then, explain “initial rate” and how to calculate the rate of thereaction using the graph.6. There are many ways to study the rate of a chemical reaction. How will the rate of the reaction in this laboratorybe measured?7. Read the general procedure, and then copy Figure 2.2 which is a visual of this procedure.8. “Assay” means measure, and a titration will be done to measure the amount of hydrogen peroxide remaining inthe beaker.

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Copy the equation on page 22 which is the titration reaction.9. The titration:a. What goes in the buret?b. What reactant in the beaker will react with the titrant?c.

Once all of the reactant is used, excess titrant added will cause the solution to turn. This is thee of the reaction.10. This is a timed experiment. What is added to stop the enzyme reaction when the time is up?

Ap Biology Lab Manual Lab 116 5

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